Abstract: Rare and endangered species, and endemic species are important to regional biological species composition. Rare and endangered species, and endemic species biodiversity research is very significant for biological conservation and maintaining the ecological environment. The rare and endangered species, and endemic species of Guilin Karst World Natural Heritage nominated area are statistical analysis based on species investigation. Conservation suggestions of rare and endangered species, endemic species are proposed in 3 levels: population, community and habitat. The results of species diversity investigation suggest that: 754 species of high plants and 525 species of vertebrate animals have been found in 16,750 ha area. Including 443 species in IUCN Species Red List, 67 species in the Conventional International Trade in Endangered Species of Wild Fauna and Flora (CITES), and 145 species in China Species Red List，and 55 species listed as China national key protected wild plants and animals. There are some relic plants, rare and endangered species with high diversity. There are 87 endemic species, which are important endemic species source. For biodiversity conservation of rare and endangered species and endemic species, population investigation and habitat analysis should be done based on level of threat of rare and endangered species. The minimum viable population and minimum dynamic area of rare and endangered species should be determined. In situ conservation or ex situ conservation is used for developing minimum viable population. Climax community conservation and monitoring research are enhanced as well as close hillsides to facilitate afforestation and community restoration project are carried on. Nature protection area is established for maintaining species survival and multiply essential habitat. Furthermore, wild orchid research and living space conservation should be enhanced as well as unique habitat conservation for cave, peak forest, cliff.