流域不同地质生态环境中水体碳酸酐酶活性特征——以桂江流域为例

申泰铭; 李为; 张强; 张阳; 张红辉; 余龙江

doi:10.3969/j.issn.1001-4810.2012.04.009

流域不同地质生态环境中水体碳酸酐酶活性特征——以桂江流域为例

doi: 10.3969/j.issn.1001-4810.2012.04.009

1.
华中科技大学生命科学与技术学院生物技术系资源生物学与生物技术研究所；分子生物物理教育部重点实验室
2.
中国地质科学院岩溶地质研究所/国土资源部、广西岩溶动力学重点实验室

基金项目: 中国地质调查局地质调查工作项目(1212011087120)

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出版历程
- 收稿日期: 2012-03-26
- 发布日期: 2012-12-25

Carbonic anhydrase activity of the water-body in different eco-environments of river basins: A case study in the Guijiang river basin

1.
Institute of Resource Biology and Biotechnology Department of Biotechnology, College of Life Science and Technology Huazhong University of Science and Technology；Key Laboratory of Molecular Biophysics, Ministry of Education
2.
Institute of Karst Geology, CAGS/ Karst Dynamics Laboratory, M LR& GZAR

摘要

摘要: 在对桂江流域进行考察时,选取了流域地质类型不同、人类活动程度不一的6条支流以及干流的水体为研究对象,研究比较了流域不同生态环境下水体中的碳酸酐酶(CA)活性、游离CO2浓度和HCO3-浓度,并对比了流域内部分区域的归一化植被指数(NDVI)数据,获得如下结果:(1)证实水体中具有CA活性,且其活性与游离CO2浓度呈负相关,与HCO3-浓度呈正相关;(2)大量的人类活动加剧流域生态环境恶化,造成植被覆盖率降低,使河流水体中的CA活性降低,HCO3-浓度降低,表明CA对岩溶作用具有不可忽视的影响;(3)流域地质类型会影响水体中CA活性,在人类活动程度相似的情况下,岩溶区水体中的CA活性高于非岩溶区。总之,在进行流域碳汇潜力调查研究时,必须综合考虑人类活动、生态环境和地质类型等因素的影响,进行科学分段调查研究与计算。CA所引起的生物岩溶作用不容忽视,其对流域碳汇的潜在贡献值得深入研究。
- 碳酸酐酶 /
- 岩溶作用 /
- 碳循环 /
- 生态环境 /
- 桂江流域
Abstract: The water-bodies in six tributaries and mainstream, which have different geologic types and different degrees of human activities, are chosen as the research object during investigating the Guijiang river basin. The carbonic anhydrase (CA) activity, free CO2 and HCO3- concentration of the water-bodies under different ecological environments are compared. The data of normalized difference vegetation index (NDVI) among some areas within the river basin are also compared. The following results are obtained :( 1) It is confirmed that the water has CA activity. Its activity is negatively correlated with free CO2 concentration and positively correlated with HCO3- concentrations; (2)Large number of human activities exacerbate the deterioration of the ecological environment in the river basin and result in vegetation cover reduction, so that the CA activity and HCO3- concentration of the water-bodies in the river decreases, indicating that the influence of CA on karst carbon sinks cannot be ignored; (3)The geological type in the river basin would affect CA activity of the water-bodies. Under the condition of similar degrees of human activity, the CA activity of the water-bodies in the karst area is higher than that in the non-karst areas. In summary, to investigate the carbon sink potential in the river basin, the impacts of human activities, eco-environments and geologic types should be comprehensively considered, and scientific segment research and calculation should be conducted. Furthermore, the biological karstification induced by CA cannot be ignored, and its contribution to the carbon sink potential deserves research.
- carbonic anhydrase (CA) /
- karstification /
- carbon cycle /
- eco-environment /
- the Guijiang river basin

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参考文献(17)

[1]	袁道先.新形势下我国岩溶研究面临的机遇和挑战［J］.中国岩溶,2009,28(4):329-331.
[2]	蒋忠诚,覃小群,曹建华,等.中国岩溶作用产生的大气CO2碳汇的分区计算［J］.中国岩溶,2011,3(4):363-367.
[3]	曹建华,袁道先,潘根兴,等.岩溶动力系统中的生物作用机理初探［J］.地学前缘,2001(3):203-209.
[4]	Smith K S, Ferry J G. Prokaryotic carbonic anhydrases［J］. FEMS Microbial Rev. 2000, 24:335-66.
[5]	Smith K S, Jakubzick C, Whittam T S, Ferry J G. Carbonic anhydrase is an ancient enzyme widespread in prokaryotes［J］. Proc Natl Acad Sci USA.1999,96:15184-15189.
[6]	Zai hua Liu. Role of carbonic anhydrase as an activator in carbonate rock dissolution and its implication for atmospheric CO2sink［J］. Acta Geologica Sinica,2001,75(3):275-278.
[7]	Wei Li, Pengpeng Zhou, Liping Jia, et al. Limestone dissolution induced by fungal mycelia, acidic materials, and carbonic anhydrase from fungi［J］. Mycopathologia,2009,167:37-46.
[8]	Wei Li, LongjiangY u, Yun Wu, et al. Enhancement of Ca2+ release from limestone by microbial extracellular carbonic anhydrase［J］. Bioresource Technology,2007,98:950-953.
[9]	Wei Li, Longjiang Yu, Qiufang Heetal. Effects of microbes and their carbonic anhydrase on Ca2+ and Mg2+ migration in column-built leached soil-limestone karst systems［J］. Applied Soil Ecology, 2005,29:274-281.
[10]	Yan Liu, Zaihua Liu, Jinliu Zhang, et al. Experimental study on the utilization of DIC by Oocystis solitaria Wittrand its influence on the precipitation of calcium carbonate in karst and non-karst waters［J］. Carbonates Evaporites,2010,25:21-26.
[11]	周秀平,黄伟军,王文圣.桂江流域径流变化特性分析［J］.广西水利水电,2008,1:22-25.
[12]	朱德浩.广西通志岩溶志［M］.南宁:广西人民出版社,2000:57-59.
[13]	Brownell P F., Bielig L M, Grof C P L. Increased carbonic anhydrase activity in leaves of sodium-deficient C4 plants［J］. Australian Journal of Plant Physiology,1991,18:589-592.
[14]	田庆久,闵祥军.植被指数研究进展［J］.地球科学进展,1998,13(4):327-333.
[15]	Wei Li, Longjiang Yu, Daoxian Yuan, et al. A study of the activity and ecological significance of carbonic anhydrase from soil and its microbes from different karste cosystems of Southwest China［J］. Plant and Soil,2005,272:133-141.
[16]	Dionisio-sese M L, Miyachi S. The effect of sodium chlorideon carbonic anhydrase activity in marine microalgae［J］. Journal of Phycology,1992,28:619-624.
[17]	何培民,徐春和,於新建,等.莱茵藻胞外碳酸酐酶分子定位与活性诱导.植物学通报,2002,19(3):317-321.