Comparative analysis on proline content in the leaves of the ancient trees in northwest of Guangxi Karst Area
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摘要: 岩溶地区具有不同于土山丘陵区的特殊生境,生长在该地区的植物具有不同的生理生态特征。采集不同树龄的樟树、大叶榕、小叶榕、桂花、重阳木、枫香、龙眼等桂西北岩溶地区常见树种的叶片,用磺基水杨酸提取—茚三酮显色法测定叶片中的脯氨酸含量。通过不同样品脯氨酸含量的比较分析,探讨脯氨酸含量与树龄、树木生存状况及其它生态因子的相关性,以便为古树生物学保护提供科学依据。结果表明:不同树种叶片的脯氨酸含量不同,不同树龄的同一树种叶片中的脯氨酸含量有差异。随着树龄的增长,叶片中的脯氨酸含量有波动,且老叶和新叶(嫩叶)的脯氨酸含量变化规律基本一致,通常表现为先升后降。叶片中的脯氨酸含量可以作为古树保护的参考指标。Abstract: The habitats of plants in karst area are different from that in the mountain and hilly land formed in clastic rocks. Special habitat brings up different group of plant. The plants in karst area have different physiological and ecological adaptability features. Different ages of leaves are collected from the common tree species, including C. Camphora, F.virens Ait.var.sublanceolata, F.microcarpa var.pusillifolia, O.fragrans.B.polycarpa, L.formosana and D.longan, which distribute in northwest Guangxi. And then proline content in the leaves is determined by the method of sulfosalicylic acid extraction-ninhydrin coloration. By comparative analysis on the proline content in different leaf-samples, correlation between proline content and tree age, the living conditions of the trees and other ecological factors are studied, so as to provide scientific basis for biological protection of the old trees. The result shows that different tree species have different proline content in their leaves; the same species leaves with different ages had different proline content. With the growth of tree-age, there are some fluctuations in proline content in the leaves. The variation of proline content in the old leaves is similar to the new ones, and the proline content usually increase at first and then decrease. Lastly, it is proposed that proline content in the leaves should be used as the reference index of for tree protection.
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Key words:
- Northwest Guangxi /
- karst area /
- old tree /
- praline
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[1] 韩冰,欧阳志云,徐卫华,等.我国西南地区物种多样性保护效果［J］.生态学报,2008,28(9):4589-4593. [2] 何学元.论云南名木古树的保护价值［J］.林业调查规划,2004,29(1):73-75. [3] 黄志平.广西古树名木资源现状及保护管理对策［J］.林业调查规划2005,30(5):112-115. [4] 莫栋材,卢树洁,梁丽华,等.广州古树名木养护复壮技术研究［J］.1995,(4):17,19-25. [5] 徐德嘉.古树名木衰败原因调查分析(古树名木复壮研究系列报告之二)［J］.苏州城建环保学院学报,1995,8(4):1-5. [6] 徐德嘉,徐向扬.土壤管理对古树名木复壮的研究(古树名木复壮研究系列报告之三)［J］.苏州城建环保学院学报,1996,9(4):1-6. [7] 袁东升,钱妙芬.环境污染对成都市古树名木的危害及防治对策研究［J］.成都信息工程学院学报,2005,20(3):336-340. [8] 朱虹,祖元刚,王文杰.逆境胁迫条件下脯氨酸对植物生长的影响［J］.东北林业大学学报,2009,37(4):86-89. [9] 蔡健,兰伟.大白菜叶游离脯氨酸含量变化的研究［J］.安徽农业科学,2006,34(12):2638-2641. [10] 职明星,李秀菊.脯氨酸测定方法的改进［J］.河南科技学院学报(自然科学版)2005,33(4):10-12. [11] 全先庆,张渝洁,单雷,等.脯氨酸在植物生长和非生物胁迫耐受中的作用［J］.生物技术通讯,2007,18(1):159-163. [12] 张成军,解恒才,郭佳秋,等.干旱对4种木本植物幼苗脯氨酸含量的影响［J］.南京林业大学学报(自然科学版),2005,29(5):33-36. [13] 全先庆,张渝洁,单雷,等.高等植物脯氨酸代谢研究进展［J］.生物技术通报,2007,(1):14-18. [14] 赵瑞雪,朱惠森,程钰宏,等.植物脯氨酸及其合成酶研究进展［J］.草业科学,2008,25(2):90-96. [15] 孙瑞莲,王文兴,周启星,等.球果蔊菜脯氨酸的积累与Cd耐性的关系［J］.中国环境科学,2009,29(2):142-146. [16] 张殿息,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸含量的方法［J］.植物生理学通讯,1990,(4):62-65. [17] 张义贤,张丽萍.重金属对大麦幼苗膜脂过氧化及脯氨酸和可溶性糖含量的影响［J］.农业环境科学学报,2006,25(4):857-860. [18] 袁有波,李继新,丁福章,等.干旱胁迫对烤烟叶片脯氨酸和可溶性蛋白质含量的影响［J］.安徽农业科学,2008,36(21):8891-8892. [19] 王晓英,黄玉清,王冬梅,等.青檀光合作用和荧光特性对岩溶区不同生境的响应［J］.林业科学研究,2009,22(4):475-481. [20] 苏维词.喀斯特区域生态系统多样性刍议［J］.中国岩溶,1996,15(3):217-223. [21] 周蓓,李艳娜,陈忠.生物多样性对岩溶地区生态保护、恢复及重建的意义［J］.山地农业生物学报,2003,22(3):248-252. [22] 吴耿,果茂腾,刘建民,等.广西弄拉几种适生药材叶片对岩溶环境的生态适应［J］.现代生物医学进展,2008,8(2):341-345. [23] 杜忠,任珺,陶玲.不同污染区对绿化树种游离脯氨酸含量的影响［J］.上海环境科学,2008,27(1):25-28. [24] 陶玲,任珺,杜忠,等.兰州市大气污染对植物中游离脯氨酸含量的影响［J］.环境化学,2008,27(4):499-502. [25] Moftah A E, Michel B E. The effect of sodium choloride on solute potential and proline accumulation in soybean leaves［J］.Physiol Plant,1987,83:238-240. [26] Hanson A D, Nelson C E, Pedersen A R,et al. Capacity for proline accumulation during water stress in barley and its implications for breeding for drought resistance［J］. Crop Sci,1979,19:489-493. -
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