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Volume 36 Issue 4
Aug.  2017
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Article Navigation >  CARSOLOGICA SINICA  > 2017  >  36(4): 572-579
MAN Baiying, CHENG Xiaoyu, WANG Hongmei, YUN Yuan, ZHONG Yuhong. Isolation and 16S rRNA gene sequence analysis of Actinomycetes from weathered rocks in Heshang Cave, central China[J]. CARSOLOGICA SINICA, 2017, 36(4): 572-579. doi: 10.11932/karst20170420
Citation: MAN Baiying, CHENG Xiaoyu, WANG Hongmei, YUN Yuan, ZHONG Yuhong. Isolation and 16S rRNA gene sequence analysis of Actinomycetes from weathered rocks in Heshang Cave, central China[J]. CARSOLOGICA SINICA, 2017, 36(4): 572-579. doi: 10.11932/karst20170420
shu

Isolation and 16S rRNA gene sequence analysis of Actinomycetes from weathered rocks in Heshang Cave, central China

doi: 10.11932/karst20170420
  • 1.

    School of Environmental Studies,China University of Geosciences/State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences

  • 2.

    School of Environmental Studies,China University of Geosciences

  • Publish Date: 2017-08-25
    Abstract
  • The specific goal of this work was to investigate the actinobacterial diversity in the weathered rocks by culture-dependent approach in Heshang Cave, Hubei, China. Three weathered rocks samples (W1, W2 and W3) were collected from aphotic zone and twilight zone in Heshang Cave with sterile centrifuge tubes in August, 2015. Five modified media and one self-designed medium of extracting of weathered rocks agar medium were used for isolation of actinomycetes in the weathered rocks of Heshang Cave. The isolates were identified by 16S rRNA gene sequencing using special primer for actinomycetes. Result shows that in total of 72 strains obtained by pure culture from 6 different media. The phylogenetic analysis showed that 72 actinomycetes sequences were classified into four genera in three orders of Actinobacteria with Streptomyces being dominative. The number of isolated strains in genera Streptomyces, Rhodococcus, Tsukamurella and Microbacterium was 69, 1, 1 and 1, respectively. Samples of W1 and W2 in aphotic zone were only isolated 9 and 10 strains respectively, while sample of W3 in twilight zone were obtained 53 strains. The results offer valuable information and isolates for the subsequent study on the interaction between carbonate and microbes.

     

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