• Included in CSCD
  • Chinese Core Journals
  • Included in WJCI Report
  • Included in Scopus, CA, DOAJ, EBSCO, JST
  • The Key Magazine of China Technology
LIU Lu, LI Fuchun, LI Lei, ZHANG Chonghong, LU Jiejie. Carbonic anhydrase excreted by bacteria induces the formation of carbonate minerals[J]. CARSOLOGICA SINICA, 2017, 36(4): 433-440. doi: 10.11932/karst20170402
Citation: LIU Lu, LI Fuchun, LI Lei, ZHANG Chonghong, LU Jiejie. Carbonic anhydrase excreted by bacteria induces the formation of carbonate minerals[J]. CARSOLOGICA SINICA, 2017, 36(4): 433-440. doi: 10.11932/karst20170402

Carbonic anhydrase excreted by bacteria induces the formation of carbonate minerals

doi: 10.11932/karst20170402
  • Publish Date: 2017-08-25
  • Study on the mechanism of carbonate mineralization in the presence of microorganisms is of great significance for the global carbon cycle, soil formation and evolution and other related issues. In order to clarify the role of extracellular carbonic anhydrase (CA) excreted by bacteria in the process of carbonate mineralization, a series of chemical experiments were carried out in the CA-calcium acetate-tryptone (without carbonate) system for 120 hours. The extracellular CA from MF-2 strains was extracted through precipitation of protein by ammonium sulfate and dissolution of protein by Tris-H2SO4 buffer solution. Synchronously, a series of control experiments without CA were performed. After the experiments, the solid and the liquid phases were separated using the centrifugation method. The mineral species, its chemical composition and morphology were characterized by X-ray diffractometer (XRD) and scanning electron microscope accompanied by energy-dispersive spectrometry (SEM/EDS). The concentration of calcium ion in the solution was determined by the inductively coupled plasma optical emission spectrometer (ICP-OES). The results show that (1) the precipitate with visible amount formed in the system with extracellular CA. With the prolongation of the reaction time, the precipitate weight increased gradually, while the Ca2+ concentration in the solution decreased. The treatment results with 30% hydrogen peroxide show that carbonate mineral was the dominant component in the precipitate. The precipitate formed in the control experiments was significantly less than that in the CA experiments, which was mainly composed of organic substances. This adequately demonstrates that the extracellular CA excreted by MF-2 strain could significantly promote precipitation of carbonate minerals. (2) No crystalline formed in the initial stage of experiments (before 48th h) with CA, and calcite was dominant mineral in the precipitate formed in the middle and late stages. This was obviously different from the incubation experiments with MF-2 strain. Only vaterite formed in the latter case. (3) The mineral morphologies formed by the action of extracellular CA were rhombohedral, spherical and hemi-spherical, in which then rhombohedral mineral dominated. This was also different from the incubation experiments with MF-2 strain (spherical and bowl-shaped in the latter case). The statistical results based on several SEM photographs show that the proportion of rhombohedral-shaped minerals decreased gradually with time, from nearly 100% at 48th h to 84% at 120th h, i.e. spherical and hemi-spherical minerals gradually increased. (4) CA accelerated hydration reaction of carbon dioxide and promoted formation of carbonate minerals. The CO2 participated in the reaction might largely come from air.

     

  • [1]
    ?Lal R. Soil carbon sequestration impacts on global climate change and food security[J]. Science, 2004, 304 (5677): 1623-1626.
    [2]
    Aquilano D, Otálora F, Pastero L, et al. Three study cases of growth morphology in minerals: Halite, calcite and gypsum [J]. Progress in Crystal Growth and Characterization of Materials, 2016, 62 (2): 227-251.
    [3]
    Hirmas D R, Amrhein C, Graham R C, et al. Spatial and processbased modeling of soil inorganic carbon storage in an arid piedmont [J]. Geoderma, 2010, 154 (3-4): 486-494.
    [4]
    Lian B, Hu Q, Chen J, et al. Carbonate biomineralization induced by soil bacterium Bacillus megaterium [J]. Geochimicaet CosmochimicaActa, 2006, 70 (22): 5522-5535.
    [5]
    Meldrum N U, Roughton F J W. Carbonic anhydrase. Its preparation and properties [J]. Journal of Physiology, 1933, 80 (80): 113-142.
    [6]
    Lindskog S. Structure and mechanism of carbonic anhydrase [J]. Pharmacology and Therapeutics, 1997, 74 (1): 1-20.
    [7]
    Liu Z, Bartlow P, Dilmore R M, et al. Production, purification, and characterization of a fusion protein of carbonic anhydrase from Neisseria gonorrhoeae and cellulose binding domain from Clostridium thermocellum [J]. Biotechnology Progress, 2009, 25 (1): 68-74.
    [8]
    Kim I G, Jo B H, Kang D G, et al. Biomineralizationbased conversion of carbon dioxide to calcium carbonate using recombinant carbonic anhydrase [J]. Chemosphere, 2012, 87 (10): 1091-1096.
    [9]
    Kanth B K, Min K, Kumari S, et al. Expression and characterization of codonoptimized carbonic anhydrase from Dunaliella species for CO2 sequestration application [J]. Applied biochemistry and biotechnology, 2012, 167 (8): 2341-2356.
    [10]
    Achal V, Pan X. Characterization of urease and carbonic anhydrase producing bacteria and their role in calcite precipitation [J]. Current microbiology, 2011, 62 (3): 894-902.
    [11]
    Dhami N K, Reddy M S, Mukherjee A. Synergistic role of bacterial urease and carbonic anhydrase in carbonate mineralization [J]. Applied biochemistry and biotechnology, 2014, 172 (5): 2552-2561.
    [12]
    李为, 曹龙, 周蓬蓬, 等. 温度对细菌碳酸酐酶催化碳酸钙沉积的影响[J]. 2013, 41(4): 371-377.
    [13]
    崔建东, 李莹, 姬晓元, 等. 静电纺丝制备中空纤维原位固定化碳酸酐酶用于二氧化碳的吸收[J]. 高等学校化学学报, 2014, 35(9): 1999-2006.
    [14]
    Xu Q L, Zhang C H, Li F C, et al. Arthrobacter sp. strain MF-2 induces high-mg calcite formation: Mechanism and implications for carbon fixation [J]. Geomicrobiology Journal, 2017, 34 (2): 157-165.
    [15]
    Li W, Chen W S, Zhou P P, et al. Influence of initial pH on the precipitation and crystal morphology of calcium carbonate induced by microbial carbonic anhydrase [J]. Colloids and Surfaces B: Biointerfaces, 2013, 102 (1):281-287.
    [16]
    鲍士旦. 土壤农化分析 (第2版)[M]. 农业出版社, 1981: 130-132.
    [17]
    Reinke L A, Moyer M J. p-Nitrophenol hydroxylation. A microsomal oxidation which is highly inducible by ethanol [J]. Drug Metabolism and Disposition, 1985, 13 (5): 548-552.
    [18]
    张道勇, 潘响亮, 张京梅. 环境因子对Synechocystis sp.钙化动力学的影响[J]. 矿物岩石地球化学通报, 2008, 27 (2): 105-111.
    [19]
    Kontoyannis C G, Vagenas N V. Calcium carbonate phase analysis using XRD and FTRaman spectroscopy[J]. The Analyst. 2000,125(2):251-255.
    [20]
    Ridgwell A, Zeebe R E. The role of the global carbonate cycle in the regulation and evolution of the Earth system [J]. Earth and Planetary Science Letters, 2005, 234 (3-4): 299-315.
    [21]
    Dupraz C, Reid R P, Braissant O, et al. Processes of carbonate precipitation in modern microbial mats [J]. Earth-Science Reviews, 2009, 96 (3): 141-162.
    [22]
    Arp G, Reimer A, Reitner J. Photosynthesis-induced biofilm calcification and calcium concentrations in Phanerozoic oceans [J]. Science, 2001, 292 (5522): 1701-1704.
    [23]
    Faridi S, Satyanarayana T. Characteristics of recombinant alpha-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1 [J]. International journal of biological macromolecules, 2016, 89: 659-668.
    [24]
    Elder I, Han S, Tu C K, et al. Activation of carbonic anhydrase II by active-site incorporation of histidine analogs [J]. Archives of Biochemistry and Biophysics. 2004, 421 (2): 283-289.
    [25]
    Power I M, Harrison A L, Dipple G M, et al. Carbon sequestration via carbonic anhydrase facilitated magnesium carbonate precipitation [J]. International Journal of Greenhouse Gas Control, 2013, 16: 145-155.
    [26]
    雷云. 球霰石型碳酸钙的研究进展 [J]. 长江大学学报, 2014 (34): 35-39.
    [27]
    李福春, 郭文文. 三种好氧细菌诱导碳酸钙矿物的形成 [J]. 南京大学学报(自然科学), 2013, 49 (6): 665-672.
    [28]
    马芳. 赖氨酸芽孢杆菌和节杆菌作用下碳酸盐矿物的形成 [D]. 南京: 南京农业大学, 2014.
  • Relative Articles

    [1]MA Chengyou, KANG Zhiqiang, ZHANG Lihao, XUAN Huiling, NONG Peijie, PAN Shufen, KONG Qiqi, ZHU Yinian, ZHU Zongqiang. Dissolution and precipitation of calcite in different water environments[J]. CARSOLOGICA SINICA, 2023, 42(1): 29-39, 51. doi: 10.11932/karst20230102
    [2]CHANG Kaiyun, WANG Zhongcheng, WEI Xiaomeng, LIU Qiumei, ZHAO Jin, ZHAO Jie, HE Xunyang. Screening and isolation of carbonic anhydrase-producing microorganisms from rocky karst habitats[J]. CARSOLOGICA SINICA, 2023, 42(6): 1202-1212. doi: 10.11932/karst20230606
    [3]KANG Weihua, CHENG Congyu, LI Wei, YU Longjiang. Review and prospect of research on the role of micro-organisms in karst carbon cycle[J]. CARSOLOGICA SINICA, 2022, 41(3): 453-464. doi: 10.11932/karst20220312
    [4]HUANG Yangyang, LI Tingyong, XIAO Siya, CHEN Chaojun, HUANG Ran, WANG Tao, WU Yao, XU Yuzhen, QIU Haiying, YANG Yan, LI Junyun. Analysis of influencing factors on mineral morphology of active speleothem[J]. CARSOLOGICA SINICA, 2022, 41(3): 488-500. doi: 10.11932/karst20220315
    [5]LI Haitao, WU Yanyou, FU Bing. Carbon sink of microalgae in karst lakes under the influence of the extracellular of carbonic anhydrase[J]. CARSOLOGICA SINICA, 2022, 41(3): 395-400, 440. doi: 10.11932/karst20220307
    [6]CHEN Jiyu, LU Zujun, HE Qiufang, LI Qiang. Characteristics of culturable bacterial communities in karst caves with different CO2 concentrations: An example of the Xueyu cave and Shuiming cave in Chongqing[J]. CARSOLOGICA SINICA, 2020, 39(2): 264-274. doi: 10.11932/karst2020y20
    [7]HUANG Binghui, LI Qiang, FANG Junjia, CAO Jianhua, JIN Zhenjiang, PENG Wenjie, LU Xiaoxuan, LIANG Yueming. Effects of CO2 concentration gradient on carbonicanhydrase of two karst microalgae[J]. CARSOLOGICA SINICA, 2018, 37(1): 91-100. doi: 10.11932/karst20180105
    [8]ZHANG Kaiyan, LI Tongjian, ZHANG Xianqiang, SUN Min. Corrosion driving effects of three epilithic mosses in the Pudding karst area, Guizhou Province[J]. CARSOLOGICA SINICA, 2017, 36(4): 441-446. doi: 10.11932/karst20170403
    [9]LIU Tianlei, CONG Chunlei, HU Dan, WANG Shijie, ZHANG Xianqiang. Carbonic anhydrase activity of six epilithic mosses and their underlying soil in the Puding karst area,Guizhou Province[J]. CARSOLOGICA SINICA, 2017, 36(2): 187-192. doi: 10.11932/karst20170205
    [10]HE Yuanyuan, LI Qiang, CAO Jianhua, LIANG Jianhong, ZHU Minjie. Spatial differentiation of soil carbonic anhydrase under different types of land use[J]. CARSOLOGICA SINICA, 2013, 32(4): 365-370.
    [11]SHEN Tai-ming, LI Wei, ZHANG Qiang, ZHANG Yang, ZHANG Hong-hui, YU Long-jiang. Carbonic anhydrase activity of the water-body in different eco-environments of river basins: A case study in the Guijiang river basin[J]. CARSOLOGICA SINICA, 2012, 31(4): 409-414. doi: 10.3969/j.issn.1001-4810.2012.04.009
    [12]ZHANG Hai-wei, CAI Yan-jun, TAN Liang-cheng. Phase composition and formation of stalagmite minerals: Indications of climate and environment[J]. CARSOLOGICA SINICA, 2010, 29(3): 222-228. doi: 10.3969/j.issn.1001-4810.2010.03.002
    [13]LI Qiang. Pondering upon the relationship between karst dynamics theory and carbonic anhydrase and polypeptide[J]. CARSOLOGICA SINICA, 2010, 29(3): 253-257. doi: 10.3969/j.issn.1001-4810.2010.03.006
    [14]YAN Zhi-wei, LIU Hui-li, ZHANG Zhi-wei. Influences of temperature and PCO2 on the solubility of calcite and dolomite[J]. CARSOLOGICA SINICA, 2009, 28(1).
    [15]YANG Xiao, LIU Zai-hua, CAO Jian-hua, ZHANG Yun-ge. Study on the relationship between photosynthesis of maize and thezinc content and carbonic anhydrase activity in karst and non-karst areas[J]. CARSOLOGICA SINICA, 2008, 27(2): 103-107. doi: 10.3969/j.issn.1001-4810.2008.02.002
    [16]YAN Zhi-wei. Influences of SO42- on the solubility of calcite and dolomite[J]. CARSOLOGICA SINICA, 2008, 27(1): 24-31. doi: 10.3969/j.issn.1001-4810.2008.01.005
    [17]LI Wei, YU Long-jiang, HE Qiu-fang, WU Yun, YU AN Dao-xian, CAO Jian-hua. MICROBES AND ITS CARBONIC ANHYDRASE IMPACT ON THE LEACHING TO CALCIUM AND MAGNESIUM ELEMENTS IN CALCAREOUS SOIL SYSTEM[J]. CARSOLOGICA SINICA, 2004, 23(1): 1-6. doi: 10.3969/j.issn.1001-4810.2004.01.001
    [18]YU Long-jiang, WU Yun, LI Wei, ZENG Xian-dong, FU Chun-hua. STUDY ON THE DRIVING EFFECTS ON LIMESTONE CORROSION BYMICROBIAL CARBONIC ANHYDRASE[J]. CARSOLOGICA SINICA, 2004, 23(3): 225-228. doi: 10.3969/j.issn.1001-4810.2004.03.008
    [19]Zhu Wenxiao, Li Po, Pan Gaochao. CLIMATE AND CO2 OF AIR IN ZHIJIN CAVE[J]. CARSOLOGICA SINICA, 1993, 12(4): 409-417.
    [20]Cheng Shouquan. FORECASTS OF CO2 CONTENT IN THE AIR OF SOME CAVES IN GUIZHOU PROVINCE[J]. CARSOLOGICA SINICA, 1992, 11(3): 240-244.
  • Cited by

    Periodical cited type(9)

    1. 易文,杜撰文,黄盎峰,刘谭剑. 胶质芽孢杆菌改良煤矸石抗风蚀性能研究. 兰州交通大学学报. 2025(01): 1-9+38 .
    2. 王鹏伟,樊恒辉,任冠洲,谢非含,张星宇,霍江茹. 碳酸酐酶对仿岩溶碳酸氢钙生成速率的影响及其作用机理. 水利与建筑工程学报. 2025(01): 118-124+199 .
    3. 李文静,贾苍琴,王贵和,向雅然. 混凝土裂缝修复技术的研究现状及进展. 混凝土与水泥制品. 2024(12): 64-69 .
    4. 杜静,曾雪玲,张洋勇,古安林. 固井自愈合水泥技术的研究现状与发展趋势. 石油化工应用. 2023(01): 12-17 .
    5. 庞彩燕,李广悦,孙静,朱家华. 一株沙福芽胞杆菌生长及其产碳酸酐酶特性. 微生物学杂志. 2022(01): 67-73 .
    6. 韩强强,路伟,姜鲁,王亚妹. 微生物菌落体系对混凝土裂缝自修复效果的影响综述. 硅酸盐通报. 2022(09): 2993-3007 .
    7. 高旭波,潘振东,龚培俐,江玉,李成城,李鸿煜. 微生物诱导碳酸盐岩沉淀过程及作用机理. 中国岩溶. 2022(03): 441-452 . 本站查看
    8. 康卫华,程从雨,李为,余龙江. 微型生物在岩溶碳循环中的作用研究回顾与展望. 中国岩溶. 2022(03): 453-464 . 本站查看
    9. 袁亮. 微生物碳酸酐酶诱导CaCO_3沉淀的影响因素及生成机理. 生物技术通报. 2020(08): 79-86 .

    Other cited types(11)

  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1706) PDF downloads(954) Cited by(20)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return