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Volume 35 Issue 6
Dec.  2016
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Article Navigation >  CARSOLOGICA SINICA  > 2016  >  35(6): 614-618
LI Haitao, WU Yanyou, ZHAO Lihua, ZHANG Kaiyan, HANG Hongtao. Application ofbidirectional labeling method to quantifying carbon utilization in microalgae[J]. CARSOLOGICA SINICA, 2016, 35(6): 614-618. doi: 10.11932/karst20160602
Citation: LI Haitao, WU Yanyou, ZHAO Lihua, ZHANG Kaiyan, HANG Hongtao. Application ofbidirectional labeling method to quantifying carbon utilization in microalgae[J]. CARSOLOGICA SINICA, 2016, 35(6): 614-618. doi: 10.11932/karst20160602
shu

Application ofbidirectional labeling method to quantifying carbon utilization in microalgae

doi: 10.11932/karst20160602
  • 1.

    School of Karst Science, Guizhou Normal University；State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences

  • 2.

    State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences

  • Publish Date: 2016-12-25
    Abstract
  • August 2015, the Chlorella pyrenoidosawas cultivated in a greenhouse with different inorganic carbons(NaH13CO3 with different δ13C values added) in the culture medium.The δ13C of inorganic carbon in the medium and the biomass of the microalgae were detected on a daily basis. In the meantime, the organic stable carbon isotope compositions of the microalgae was also measured. The proportion of the added inorganic carbon used by microalgae was quantified by comparing their stable carbon isotope compositions using the bidirectional labeling method (NaH13CO3 with different δ13C values was added).This study compared respectively both inorganic carbon and organic carbon of the stable carbon isotope compositions. The results are as follows, it is 0.19 under 5.0 mmol/L NaHCO3, 0.37 under 10.0 mmol/L NaHCO3, and 0.57 under 20.0 mmol/L NaHCO3. At last, we analyzed the two methods for quantifying the carbon sources in algae. It is very important to calculate the proportion of different inorganic carbon in the carbon cycle research in karst lakes.

     

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