Application ofbidirectional labeling method to quantifying carbon utilization in microalgae

LI Haitao; WU Yanyou; ZHAO Lihua; ZHANG Kaiyan; HANG Hongtao

doi:10.11932/karst20160602

Volume 35 Issue 6

Dec. 2016

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Article Navigation > CARSOLOGICA SINICA > 2016 > 35(6): 614-618

LI Haitao, WU Yanyou, ZHAO Lihua, ZHANG Kaiyan, HANG Hongtao. Application ofbidirectional labeling method to quantifying carbon utilization in microalgae[J]. CARSOLOGICA SINICA, 2016, 35(6): 614-618. doi: 10.11932/karst20160602

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LI Haitao, WU Yanyou, ZHAO Lihua, ZHANG Kaiyan, HANG Hongtao. Application ofbidirectional labeling method to quantifying carbon utilization in microalgae[J]. CARSOLOGICA SINICA, 2016, 35(6): 614-618. doi: 10.11932/karst20160602

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Application ofbidirectional labeling method to quantifying carbon utilization in microalgae

doi: 10.11932/karst20160602

1.
School of Karst Science, Guizhou Normal University；State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences
2.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences

Publish Date: 2016-12-25

Abstract

Abstract

August 2015, the Chlorella pyrenoidosawas cultivated in a greenhouse with different inorganic carbons(NaH13CO3 with different δ13C values added) in the culture medium.The δ13C of inorganic carbon in the medium and the biomass of the microalgae were detected on a daily basis. In the meantime, the organic stable carbon isotope compositions of the microalgae was also measured. The proportion of the added inorganic carbon used by microalgae was quantified by comparing their stable carbon isotope compositions using the bidirectional labeling method (NaH13CO3 with different δ13C values was added).This study compared respectively both inorganic carbon and organic carbon of the stable carbon isotope compositions. The results are as follows, it is 0.19 under 5.0 mmol/L NaHCO3, 0.37 under 10.0 mmol/L NaHCO3, and 0.57 under 20.0 mmol/L NaHCO3. At last, we analyzed the two methods for quantifying the carbon sources in algae. It is very important to calculate the proportion of different inorganic carbon in the carbon cycle research in karst lakes.
- inorganic carbon utilization,
- stable carbon isotope,
- microalgae

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Application ofbidirectional labeling method to quantifying carbon utilization in microalgae

doi: 10.11932/karst20160602

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Application ofbidirectional labeling method to quantifying carbon utilization in microalgae

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