微藻CO2同化过程的稳定碳同位素分馏值

赵丽华; 吴沿友; 谢腾祥; 李海涛; 张开艳; 杭洪涛

doi:10.11932/karst20160402

微藻CO2同化过程的稳定碳同位素分馏值

doi: 10.11932/karst20160402

1.
中国科学院地球化学研究所环境地球化学国家重点实验室；中国科学院大学地球科学学院
2.
中国科学院地球化学研究所环境地球化学国家重点实验室

基金项目: 国家重点基础研究发展计划（973）专题“岩溶植被及微藻碳源利用策略及份额估算（2013CB956701）”

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出版历程
- 发布日期: 2016-08-25

Stable carbon isotope fractionation (δ13C) of microalgae on CO2 assimilation

1.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry，Chinese Academy of Sciences；College of Earth Sciences，University of Chinese Academy of Sciences
2.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry，Chinese Academy of Sciences

摘要

摘要: 研究微藻CO2同化过程中的碳同位素分馏值，对研究微藻在碳效应过程中的作用具有重要意义。目前还没有获取微藻CO2同化过程的稳定碳同位素分馏值的在体实验方法。文章以莱茵衣藻、蛋白核小球藻、及野外红枫湖混合藻三种岩溶湖泊微藻为例，利用双向标记建立了能在体获得此分馏值的方法，并通过此分馏值定量了微藻的各种无机碳利用途径份额。获得各自CO2同化过程中的碳同位素分馏值分别为15.3‰、14.8‰、21.7‰。三种藻分馏值差异主要与藻的种类及其细胞体积大小有关。利用此分馏值计算出衣藻、小球藻、混合藻自然培养下的碳酸氢根离子途径利用份额分别为100%、81.1%、97.8%，表明了生长在岩溶湖泊的微藻利用无机碳的途径主要为碳酸氢根离子。
- 微藻 /
- 稳定碳同位素分馏值 /
- 无机碳利用 /
- 碳酸氢根离子
Abstract: A study on the stable carbon isotopic fractionation (δ13C) in the process of CO2 assimilation of microalgae is very significant to investigate the role of microalgae in estimate of potential carbon dioxide (CO2) sink. However, till now it is yet unknown how to measure the fractionation on CO2 assimilation of microalgae in vivo. Bidirectional tracers, which two types of sodium bicarbonate have different δ13C-PDB values, are used as the carbon isotope labeling to establish the method of calculation of the fractionation value in this study. Furthermore, the different proportions of metabolic pathways of inorganic carbon utilization are quantified by the fractionation value in microalgae. The carbon isotope fractionation (δ13C) value of three types of algae, Chlamydomonas reinhardtii (CR), Chlorella pyrenoidosa(CP), and compound algae collected from surface water of Hongfeng Lake, are 15.3‰, 14.8‰, and 21.7‰, respectively in this paper. The difference of the fractionation values are closely related to cell size of these microalgaes. That means, the bigger cell size of microalgae, the greater the resistance produces and the longer the reaction time of process in the CO2 assimilation. This leads to greater fractional values. The percent share of bicarbonate ions calculated by the fractionation values are 100%, 81.1% and 97.8% respectively in Chlamydomonas reinhardtii, Chlorella pyrenoidosa and compound algae. The results demonstrate that the main pathway of inorganic carbon utilization of microalgae is the bicarbonate ions in karst lakes.
- microalgae /
- stable carbon isotope fractionation /
- bicarbonate ions utilization /
- bicarbonate ion

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