农业旅游活动对岩溶地区土壤微生物群落结构的影响——以重庆市铜梁区黄桷门奇彩梦园为例

王 帅; 王 洁; 骆云中; 谢德体; 高 明; 赵振洋; 程 杨; 蔡洋洋

doi:10.11932/karst20150610

农业旅游活动对岩溶地区土壤微生物群落结构的影响——以重庆市铜梁区黄桷门奇彩梦园为例

doi: 10.11932/karst20150610

1.
西南大学资源环境学院/三峡库区农业面源污染控制研究中心
2.
西南大学资源环境学院/中国科学院南京土壤研究所
3.
西南大学资源环境学院
4.
西南大学地理科学学院

基金项目: 国家“乡村土地流转与资源整合关键技术与示范”课题（2013BAJ11B02）；国家十二五科技支撑项目（2012BAD141318）

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出版历程
- 发布日期: 2015-12-25

Impact of agricultural tourism activities on microbial community structure of soil in karst area：A case research of Tongliang district of Chongqing Huangjuemen Qicaimengyuan

1.
College of Resources and Environment, Southwest University/Agricultural Nonpoint Source Pollution Control Engineering Technology Research Center in the Three Gorges Reservoir
2.
College of Resources and Environment, Southwest University/Institute of Soil Science, Chinese Academy of Sciences
3.
College of Resources and Environment, Southwest University
4.
School of Geographical Sciences, Southwest University

摘要

摘要: 开展农业旅游活动对土壤生态环境影响的专题研究，可为休闲农业旅游区的合理开发和有效保护提供理论基础，为评估未来农业旅游活动对环境的影响提供科学依据。本文以重庆市铜梁区黄桷门奇彩梦园为例，通过对游步道两侧的土壤进行调查采样，并运用磷脂脂肪酸（PLFAs）谱图分析法，研究了土壤微生物群落组成、受农业旅游活动影响的距离及其受冲击程度。结果显示：微生物群落结构受冲击范围在4 m以内（p<0.01）,微生物生物量在土壤中的分布随着离游径的距离变小而明显下降趋势；在受农业旅游活动冲击的范围内细菌、革兰氏阳性细菌、革兰氏阴性菌、真菌的生物量随着离游径的距离变小而明显下降，放线菌在部分样区表现出该规律，原生动物无此规律；对所测PLFAs数据进行多样性和冲击指数分析后得出假单胞菌（16:0）受影响程度最大，节杆菌（17:0）受影响程度最小，受冲击程度大小与该微生物在生物总量中所占的比重呈正相关；农业旅游活动对土壤微生物群落结构造成了破坏，土壤生态系统的稳定性已受到冲击。
- 岩溶地区 /
- 农业旅游活动 /
- 土壤微生物群落结构
Abstract: To conduct the matic studies on the impact of agricultural tourism on the ecological environment of soil can provide a theoretical basis for rational development of leisure agriculture tourism and provide a scientific basis for assessing the impact of future agri-tourism activities on the environment.For this study, soil samples were collected on both sides of tour trail in Qicaimengyuan of TongLiang Huangjuemen. Through field investigation, sampling and sample analysis by using phospholipid fatty acid spectrum graph method (PLFAs), the community &composition of soil microorganisms, impact range of agri-tourism and its degree of impact were investigated in this study.The results show that impact range on microbial community structure falls within 4 m (p<0.01),while the soil microbial biomass clearly declines in the direction to the tour trails. Within the scope of that impact of agricultural tourism,the similar tendency between the biomass and touring range commonly occurs in bacteria, Gram-positive bacteria, Gram-negative bacteria and fungi with the distance from the smaller trails have a clear downward trend.This biomass decline also happens in actinomycetes in some areas, but not in protozoa.The analytical results of biodiversity and impact indexes,with data derived from PLFAs analysis, show that Burkholderia bacteria (19: 0 cyclo w8c) is most likely to be affected by the tourism activity, whereas arthrobacter [18: 3 w6c (6,9,12)] is the least affected.And the impact degree is closely related to the percentage of microorganism in the total biomass.The research indicates that agri-tourism activities have caused damage to soil microbial community structure and have affected soil ecological stability.
- karst region /
- agricultural tourism /
- soil microbial community structure

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参考文献(28)

[1]	谭周进,肖启明,祖智波.旅游踩踏对张家界国家森林公园土壤微生物区系及活性的影响［J］.土壤学报,2007,1(44):184-187.
[2]	何荣,王国兵,杜家社,等.武夷山不同海拔植被土壤微生物量的季节动态及主要影响因子［J］.生态学杂志,2009,28(3):394-399.
[3]	刘纯,刘延坤,金光泽.小兴安岭6种森林类型土壤微生物量的季节变化特征［J］.生态学报,2014,34(2):451-459.
[4]	赵辉,赵铭钦,程玉渊,等.土壤微生物影响因子研究综述［J］.江西农业学报,2009,21(12):52-56.
[5]	Yergeau E,Newsham K K,Pearce D A, et al. Patterns of bacterial diversity across a range of Antarctic terrestrial habitats［J］. Environmental microbiology,2007,9(11):2670-2682.
[6]	Lauber C L,Hamady M,Knight R,et al.Pyrosequencingbased assessment of soil pH as a predictor of soil bacterial community structure at the continental scale［J］.Applied and environmental microbiology,2009,75(15):5111-5120.
[7]	Berg G,Smalla K. Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere［J］.FEMS microbiology ecology,2009,68(1):1-13.
[8]	Fierer N, Jackson R B. The diversity and biogeography of soil bacterial communities［J］.Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(3):626-631.
[9]	Andres-Abellan M,DEL áLAMO J B,Landete-Castillejos T,et al.Impacts of visitors on soil and vegetation of the recreational area“Nacimiento del Río Mundo”(Castilla-La Mancha,Spain)［J］.Environmental Monitoring and Assessment,2005,101(3): 55-67.
[10]	刘德秀,秦远好,谢德体.游憩活动对缙云山自然保护区土壤环境的影响［J］.西南农业大学学报(自然科学版),2006,28(5): 714-721.
[11]	韦大方,方凤满,杨仲元.黄山风景区步道土壤理化性质变化特征分析［J］.水土保持研究, 2012,19(2):175-179.
[12]	席建超,胡传东,武国柱,等.六盘山生态旅游区旅游步道对人类践踏干扰的响应研究［J］.自然资源学报,2008,23(2):274-284.
[13]	Frosteg?rd ?,B??th E,Tunlio A.Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis［J］.Soil Biology and Biochemistry,1993, 25(6):723-730.
[14]	Federle T W.Microbial distribution in the soil-new techniques.In Perspective in Microbial ecology.Slovene Society for Microbialogy,Ljwbljana,1986:493-498.
[15]	Frosteg?rd ? ,B??th E.The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil［J］.Biology and Fertility of Soils,1996, 22(1-2):59-65.
[16]	O’leary W M, Wilkinson S G. Gram-positive bacteria［J］. Microbial lipids,1988,1:117-201.
[17]	Zogg G P, Zak D R, Ringelberg D B, et al.Compositional and functional shifts in microbial communities due to soil warming［J］.Soil science society of america journal,1997, 61(2):475-481.
[18]	Steinberger Y, Zelles L, Bai Q Y, et al. Phospholipid fatty acid profiles as indicators for the microbial community structure in soils along a climatic transect in the Judean Desert［J］. Biology & Fertility of Soils, 1999, 28(3):292-300.
[19]	Ratledge C,Wilkinson S G.Microbial lipids［M］. London: Academic Press, 1988.
[20]	elles L ,. Identification of single cultured micro-organisms based on their whole-community fatty acid profiles, using an extended extraction procedure［J］. Chemosphere, 1999, 39(4):665-682.
[21]	杨文浩.镉污染/镉-锌-铅复合污染土壤植物提取修复的根际微生态效应研究［D］.杭州:浙江大学,2014:44.
[22]	石强,雷相东,谢红政.旅游干扰对张家界森林公园土壤影响的研究［J］.四川林业科技,2010,23(3):28-33.
[23]	戈峰.现代生态学［M］.北京:科学出版社, 2002: 252-254.
[24]	沈利娜,邓新辉,蒋忠诚,等.不同植被演替阶段的岩溶土壤微生物特征:以广西马山弄拉峰丛洼地为例［J］.中国岩溶,2007,26(4):310-314.
[25]	彭伶俐,王琴,辛明秀.自然界中不可培养微生物的研究进展［J］.微生物学杂志,2011,31(2):75-79.
[26]	谢龙莲,陈秋波,王真辉,等.环境变化对土壤微生物的影响［J］.热带农业科学,2004,24(3):39-41.
[27]	齐鸿雁,薛凯,张洪勋.磷脂脂肪酸谱图分析方法及其在微生物生态学领域的应用［J］.生态学报,2003,23(8):1576-1582.
[28]	张秋芳,刘波,林营志,等.土壤微生物群落磷脂脂肪酸PLFA生物标记多样性［J］.生态学报,2009,29(8):4127-4134.