样品采集和保存方法对水中溶解无机碳同位素分馏的影响

杨 会; 王 华; 吴 夏; 唐 伟; 涂林玲; 应启和

doi:10.11932/karst201506014

样品采集和保存方法对水中溶解无机碳同位素分馏的影响

doi: 10.11932/karst201506014

中国地质科学院岩溶地质研究所/国土资源部、广西壮族自治区岩溶动力学重点实验室

基金项目: 广西科技创新能力与条件建设计划项目（桂科能14123006-26）；国土资源部公益性行业科研专项（201411075）；中国地质调查局项目（12120113005200）；广西青年科学基金项目（2014GXNSFBA118227）；国家自然科学基金（441501222）

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出版历程
- 发布日期: 2015-12-25

The influence of different pretreatment methods on theδ13C value of dissolved inorganic carbon in water

Institute of Karst Geology, CAGS/Key Laboratory of Karst Dynamic, MLR & GZAR

摘要

摘要: 为探明不同的样品前处理方法对水中溶解无机碳同位素的影响，寻找最佳的样品前处理方法，本文系统研究了不同样品采集方法（顶空样品瓶和聚乙烯瓶）和保存方法（不同的温度和是否加饱和氯化汞）以及储存时间对不同类型水样溶解无机碳同位素值的影响。结果表明：在水样类型相同的情况下，顶空瓶法较聚乙烯瓶法采集的水样δ13CDIC值更稳定；用聚乙烯瓶采集水样，加入饱和氯化汞能抑制微生物生长，不加饱和氯化汞的水样δ13CDIC值变化无规律且幅度大，而加了饱和氯化汞的水样δ13CDIC值更接近初始值，但随保存温度的升高和保存时间的推移，其δ13CDIC值逐渐偏重，主要受温度升高有利于HCO3-水解并释放CO2的影响；不同类型水样在不加饱和氯化汞条件下，随保存时间加长，岩溶水的δ13CDIC值较初始值偏重，混合水和非岩溶区水δ13CDIC值较初始值偏轻。实验表明，用顶空样品瓶采集的水样保存100天，δ13CDIC最大变化值为0.42‰；用顶空样品瓶采集的水样保存时间长，δ13CDIC值变化小，最能反映样品的初始值，是最佳的样品前处理方法。用聚乙烯瓶采集的水样加饱和氯化汞并保存在5 ℃能满足样品短时间的保存。
- 溶解无机碳同位素 /
- 同位素分馏 /
- 前处理条件 /
- 岩溶地区
Abstract: In order to research the influence of different sample preparation methods on the dissolved inorganic carbon isotope in water samples, and to establish the best sample preparation method, this paper systematically study the sample collection (headspace vial and polyethylene bottle) methods, storage temperature, saturation mercuric chloride and storage duration which affect the dissolved inorganic carbon isotope value in different types of water samples. The results show that δ13CDIC headspace vial collection method is more stable than the polyethylene bottle collection method for the same water type. Using polyethylene bottle collection method and adding saturated mercury chloride may inhibit the growth of microorganisms. The δ13CDIC value irregularly varies in a big range in case of no saturated mercury chloride added. In contrast, the δ13CDIC value roughly stabilizes at its initial value. The value increased in the case of the relative high temperature or long duration, which is mainly attributed to the release of CO2 in this condition of sample preservation. Different types of water samples without saturated mercuric chloride and with prolonged preservation, the karst water δ13CDIC was more positive than the original value, and the mixed water and non- karst water was negative than the original value. The experiment results show, the maximum difference of δ13C value was merely 0.42‰ after 100 days of storage by using headspace vial sample collection, which is proved to be the best way of sample collection and preservation. In the meantime, the water sampled by polyethylene bottle collection method and stored in a 5 ℃ condition can last a short time to maintain the reliable δ13CDIC value.
- dissolved inorganic carbon isotope /
- isotope fractionation /
- pretreatment conditions /
- karst area

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