Cd2+胁迫下小球藻(Chlorella vulgaris)对岩溶水HCO3-、Ca2+利用研究——以桂林寨底地下河为例

胡清菁; 王 培; 华 磊; 曹建华; 李 强

Cd2+胁迫下小球藻(Chlorella vulgaris)对岩溶水HCO3-、Ca2+利用研究——以桂林寨底地下河为例

1.
中国地质科学院岩溶地质研究所/国土资源部、广西壮族自治区岩溶动力学重点实验室；广西大学环境学院
2.
中国地质科学院岩溶地质研究所/国土资源部、广西壮族自治区岩溶动力学重点实验室；中国地质大学地球科学与资源学院
3.
荆州水务集团有限公司
4.
中国地质科学院岩溶地质研究所/国土资源部、广西壮族自治区岩溶动力学重点实验室

基金项目: 中国地质调查局项目：岩溶地区固碳增汇试验示范（12120113005300）；广西自然科学基金（2010GXNSFB013004）

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出版历程
- 收稿日期: 2014-03-03
- 发布日期: 2014-06-25

Utilization of karst water HCO3-, Ca2+ by Chlorella vulgaris under different Cd2+ concentrations stress

1.
Institute of Karst Geology, CAGS/Key Laboratory of Karst Dynamics,MLR & GZAR；School of Environment, Guangxi University
2.
Institute of Karst Geology, CAGS/Key Laboratory of Karst Dynamics,MLR & GZAR；School of the Earth Sciences and Resources, China University of Geosciences (Beijing)
3.
Jingzhou Water Affairs Group Company Limited
4.
Institute of Karst Geology, CAGS/Key Laboratory of Karst Dynamics,MLR & GZAR

摘要

摘要: 初级生产者藻类对维持生态系统稳定具有重要的意义。2012年底广西龙江重金属Cd2+污染对其下游水体中水生生物造成了严重的影响，为了解Cd2+对岩溶水体中藻类碳汇效应的影响，针对广西龙江重金属Cd2+污染，文章通过室内封闭培养体系研究了在0、10、20、40 μmol/L不同Cd2+浓度胁迫下，小球藻对岩溶水中游离CO2、HCO3-和Ca2+的利用情况以及体系中pH和生物量的相应变化。结果表明：当Cd2+浓度在0～10 μmol/L时，小球藻对岩溶水中Ca2+和HCO3-的利用基本上没有受到影响；当Cd2+浓度在10～40 μmol/L时，对小球藻利用Ca2+和HCO3-具有一定的抑制作用；当Cd2+浓度高于40 μmol/L时，小球藻将不能利用岩溶水中Ca2+和HCO3-。同时pH漂移实验表明：当Cd2+浓度在0～20 μmol/L时，小球藻能同时利用岩溶水中游离CO2和HCO3-进行光合作用；Cd2+浓度为10 μmol/L时，体系中藻细胞生物量与空白对照组基本相同；当Cd2+浓度在20～40 μmol/L时，小球藻只能利用岩溶水中游离CO2进行光合作用；当Cd2+浓度为20 μmol/L时，藻细胞生物量为空白对照组的一半；当Cd2+浓度为40 μmol/L时，小球藻生物量仅为20 μmol/L时的一半。
- 岩溶水 /
- 小球藻 /
- Cd2+胁迫 /
- pH漂移技术 /
- 碳汇
Abstract: The carbon sink effect of algae is a hot topic in global carbon sink research. At the end of 2012, the Longjiang river cadmium pollution event greatly harmed downstream aquatic life. In order to study the effect of cadmium pollution on the algae carbon sink effect, this experiment explored the HCO3- and Ca2+ in karst water used by Chlorella vulgaris cultivated in a closed system under the stress of different Cd2+ concentrations (0, 10, 20, 40 μmol/L), as well as changes to pH and the biomass of Chlorella vulgaris in the system. The results indicated that HCO3- and Ca2+ utilization by Chlorella vulgaris photosynthesis is essentially unchanged when the Cd2+ concentration is in the range of 0 μmol/L to 10 μmol/L. When the Cd2+ concentration is 10-40 μmol/L, the use of HCO3- and Ca2+ in karst water by Chlorella vulgaris is inhibited. When the Cd2+ concentration exceeds 40 μmol/L, Chlorella vulgaris essentially doesn’tuse the HCO3- and Ca2+ in the closed system. Furthermore, pH drift experiments show that when the Cd2+ concentration is in the 0 μmol/L to 20 μmol/L range, Chlorella vulgaris can simultaneously use the free CO2 and HCO3- in karst water for photosynthesis. But when the Cd2+ concentration is between 20 μmol/L and 40 μmol/L, Chlorella vulgaris only uses free CO2 as the carbon source. Therefore, the cadmium pollution in karst aquatic ecosystem has a pronounced impact on the algae carbon sink. 
- karst water /
- Chlorella vulgaris /
- stress of different Cd2+ concentrations /
- pH drift /
- carbon sink

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